Enzo Life Sciences DNA损伤酶联免疫检测试剂盒DNA damage ELISA kit
——用于肿瘤、细胞凋亡和氧化应激研究中DNA损伤的快速检测
细胞暴露于氧化和环境应激经常导致基因组DNA的分解或氧化，评价基因组DNA完整性或评估氧化DNA存在的测定法经常用作验证凋亡或DNA损伤开始的手段。8-羟基-2 '-脱氧鸟苷(8-OHdG)是一种修饰的核苷碱基，是氧化DNA损伤和氧化应激的常用生物标志物。尿液中的8-OHdG及其类似物8-羟基鸟苷和8-羟基鸟嘌呤与许多退行性疾病有关。在许多疾病中，包括膀胱癌和前列腺癌，囊性纤维化，特应性皮炎和类风湿性关节炎中，已经研究了ROS的相关性和使用8-OHdG作为氧化应激的生物标志物。帕金森氏病、阿尔茨海默氏病和亨廷顿氏病是神经退行性疾病，被认为是由具有这些疾病遗传倾向的人接触神经毒素引起的。氧化应激与这些疾病的发病机制有关，在多种神经系统疾病中检测到DNA损伤水平升高。
Enzo Life Sciences的DNA损伤酶联免疫检测试剂盒是一种快速灵敏的竞争法免疫检测试剂盒，可用于检测和定量尿液、血清和唾液样品中的8-羟基-2 '-脱氧鸟苷(8- OHdG)。
产品特点
● 高灵敏度，可检测低至1 ng/ml 8-OHdG
● 在多类型样本中验证并获得可靠结果
● 优于半定量Western blot分析的全定量检测
● 无边缘效应，每个试剂盒可检测更多样本
产品详情
|
产品货号 |
ADI-EKS-350 |
|
产品名称 |
DNA damage ELISA kit（DNA损伤ELISA试剂盒） |
|
规格 |
96 wells |
|
灵敏度 |
0.59 ng/ml |
|
检测范围 |
0.94 - 60 ng/ml |
|
检测时间 |
<2.5 hours |
|
适用样本类型 |
血清，唾液，尿液（有文献报道血浆、精液、细胞裂解物、培养上清液和DNA提取物） |
|
适用种属 |
不限物种 |
|
交叉反应 |
The DNA Damage ELISA also detects 8-hydroxyguanosine (product of oxidative RNA damage) and 8-hydroxyguanine (product of oxidative DNA damage by hydroxyl radicals) |
|
试剂盒组分 |
Microtiter plate, Standard, Sample diluent, Wash buffer concentrate, Antibody, Antibody diluent, Conjugate, Conjugate diluent, TMB Substrate, Stop solution 2 |
标准曲线示例
部分产品文献引用
1. The Dietary Fermentable Fiber Inulin Alters the Intestinal Microbiome and Improves Chronic Kidney Disease Mineral-Bone Disorder in a Rat Model of CKD: A. Biruete, et al.; bioRxiv (2023)
2. Octanoic Acid - An Insecticidal Metabolite of Conidiobolus coronatus (Entomopthorales) That Affects Two Majors Antifungal Protection Systems in Galleria mellonella (Lepidoptera): Cuticular Lipids and Hemocyte: A. Kaczmarek, et al.; Int. J. Mol. Sci. 23, 5204 (2022)
3. Wasp Venom Ameliorates Scopolamine-Induced Learning and Memory Impairment in Mice: J.H. Chae, et al.; Toxins 14, 256 (2022)
4. Arbutin protects brain against middle cerebral artery occlusion-reperfusion (MCAo/R) injury: M. Kumar, et al.; Biochem. Biophys. Res. Commun. 577, 52 (2021)
5. Berberine ameliorates renal injury in a rat model of D-galactose-induced aging through a PTEN/Akt-dependent mechanism: H.E. El-Horany, et al.; Arch. Physiol. Biochem. 126, 157 (2020), Application(s): Rat blood serum
6. Evaluation of Effectiveness of Ultraviolet Emitting Lamps on the Cutaneous Production of Vitamin D3: Relationship of the Lamps Vitamin D3 Producing Potential to the Production of 8-Hydroxy-2'-Deoxyguanosine and Nitric Oxide: A. Shirvani, et al.; Anticancer Res. 40, 565 (2020)
7. In Vivo Anti-Inflammatory Potential of Viscozyme®-Treated Jujube Fruit: Y. Kim, et al.; Foods 9, 1033 (2020), Application(s): Mouse Liver tissue homogenate
8. Inhibitory Effect of Steamed Soybean Wastewater Against DSS-Induced Intestinal Inflammation in Mice: S. Jeong, et al.; Foods 9, 954 (2020), Application(s): Quantification of 8-OHdG level in mouse plasma.
9. N-acetylcysteine (NAC), an anti-oxidant, does not improve bone mechanical properties in a rat model of progressive chronic kidney disease-mineral bone disorder: M.R. Allen, et al.; PLoS One 15, e0230379 (2020)
10. Neuroprotective Effects of Euonymus alatus Extract on Scopolamine-Induced Memory Deficits in Mice: Y. Woo, et al.; Antioxidants (Basel) 9, 449 (2020), Application(s): Serum
11. Antioxidative effects of olive polyphenols compared to vitamin E in piglets fed a diet rich in N-3 PUFA: J. Leskovec, et al.; Animals (Basel) 9, 161 (2019), Application(s): ELISA using pig serum
12. MicroRNA-204-3p Attenuates High Glucose-Induced MPC5 Podocytes Apoptosis by Targeting Braykinin B2 Receptor: X. Han, et al.; Exp. Clin. Endocrinol. Diabetes 127, 387 (2019), Application(s): Mouse MPC5 podocyte lysates
13. Oxidative stress and mitochondrial responses to stress exposure suggest that king penguins are naturally equipped to resist stress: A. Stier, et al.; Sci. Rep. 9, 8545 (2019), Application(s): Penguin plasma and red blood cell lysate
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