北京时间5月11日，浙江万里学院葛楚天团队在 Science 杂志上发表了题为 The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species （IF 37.205）研究论文，关于红耳龟温度依赖型性别决定研究领域取得突破性进展，解开了温度依赖型性别决定研究长达半个世纪的谜团。

葛教授研究团队一直使用联科生物产品，对联科（Multi Sciences）这一民族品牌给予了高度评价和信赖。此次突破性研究当中使用的联科Maxi ECL溶液是一种新型配方的级辣根过氧化物酶(HRP)底物化学发光液，具有超高灵敏度、性能稳定、半衰期长、背景(本底)较低、重复性好等优点。它可用于固相膜(如 NC、PVDF)上结合的蛋白(Western Blot)或核酸(Southern/Northern Blot)的检测，既可感光X光胶片，也适用于荧光CCD相机的荧光扫描。

联科生物是拥有自主知识产权和核心**技术的研发生产型企业。十六年来专注于为科研工作者提供种类全面的细胞生物学、分子生物学产品和专业的检测技术服务。联科旗下拥有以“ELISA检测试剂盒”、“流式抗体”、“Western检测”、“分子生物学试剂”等为代表的超过400000种科研试剂产品，持有5项国家技术发明**，生产体系通过ISO9001国际质量体系认证，累计服务超过50000名全球满意客户，超过20000篇标注使用联科产品的论文发表于包括Science、Nature等国际一流学术期刊。

葛楚天教授文章内容摘要：

The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species

Chutian Ge,1*† Jian Ye,2* Ceri Weber,3 Wei Sun,1 Haiyan Zhang,1 Yingjie Zhou,1 Cheng Cai,1 Guoying Qian,1† Blanche Capel3†

Temperature-dependent sex determination is a notable model of phenotypic plasticity. In many reptiles, including the red-eared slider turtle Trachemys scripta elegans (T. scripta), the individual’s sex is determined by the ambient temperature during egg incubation. In this study, we show that the histone H3 lysine 27 (H3K27) demethylase KDM6B exhibits temperature dependent sexually dimorphic expression in early T. scripta embryos before the gonad is distinct. Knockdown of Kdm6b at 26°C (a temperature at which all offspring develop into males) triggers male-to-female sex reversal in >80% of surviving embryos. KDM6B directly promotes the transcription of the male sex-determining gene Dmrt1 by eliminating the trimethylation of H3K27 near its promoter. Additionally, overexpression of Dmrt1 is sufficient to rescue the sex reversal induced by disruption of Kdm6b.This study establishes causality and a direct genetic link between epigenetic mechanisms and temperature-dependent sex determination in a turtle species.

文章详细信息请参阅：文件1 文件2

化学发光显色试剂盒：

► 高灵敏度的非放射性检测系统：可检测低至100fg～1pg的抗原，适用于痕量抗原的检测。

► 高分辨率：本底极低，即使长时间曝光，背景仍然极低，远远优于国产与进口同类产品

► 半衰期长：信号衰减慢，8个小时以上仍可感光X光胶片。

不同ECL试剂盒的选择：